This study aims to analyze the use of Instructional Media for Differentiated instruction by English teachers at SMPN 6 Purwokerto. Through observational studies, interviews, and a thorough review of lesson plans, it was determined that two English teachers employed Instructional Media in alignment with students' readiness, interest, and learning profiles. In the use of Instructional Media, the teachers arrange and select Instructional Media by adapt to learning materials, student needs and ease of access to adapted to the principles of Differentiated Instruction. In using Instructional Media in the classroom, Instructional Media is used as a communication tool to deliver information from teachers to students with its function to deliver learning materials. Notwithstanding the implementation of instructional media, several impediments were identified, including a paucity of student discipline, lack of using instructional technologies and inadequate facilities that hindered the effective integration of instructional strategies.